Car rental at malaga international airport Dulhunty, A
Organization of Ca^sup 2+^ Divulge Units in Excitable Silky Brawn of the Guinea-Pig Urinary BladderABSTRACT
malaga airport cars Unveiling
This
work doesn't address the question of pharmacomechanical coupling, although this substitution Ca^sup 2+^ divulge appliances is well shown within the guinea pig detrusor brawn.
malaga car hire MATERIALS And techniques
Guinea pigs of either sexuality (~300g) were murdered by cervical dislocation pursued by bleeding.
Western blotting
A crude membrane preparation was extracted from a homogenate of whole guinea pig bladders by pelleting at 30,000 × g the supernatant from a preliminary 14,000 × g centrifugation. Membranes from inside the high KCL eradication of bunnie skeletal brawn and from bunnie neonate urinary bladder and tummy arrangements were used as regulates. 50 µ-g of the skeletal brawn membranes and 150 µg of numerous bladder fractions were broke up on SDSPAGE and dim with Amido-black discoloration, afterwards exchange to PVDF rigid help. The blot was probed with Sheep6 Anti-DHPR 1:500 (Pragnell et al., 1991; Arikkalh et al., 2003) and a bunnie anti-sheep IgG peroxidase at 1:5000 dilution as subsidiary antibody.
Three-d immunofluorescence
Electron microscopy
SR capacities were regained utilizing a point-counting morphometric approach (Weibel, 1979) from eight guinea-pig urinary bladders, three tissue-blocks for each animal, with three sections for each block and 7 snap shots for each part. Low magnifications were utilized for cellular, extracellular space and mitochondria and taller magnifications (25,000) for the contribution of mitochondria, caveolae, and SR. The level portions of the cellular organelles were computed from inside the quotients of caveolae/mitochondria, central SR/mitochondria and peripheral SR/mitochondria and even mitochondria/cell, afterwards subtraction of the extracellular space. SR was thought out peripheral if its membranes were situated in a distance <80 nm="" from="" inside="" the="" sarcolemma.="" in="" tangential="" parts="" of="" the="" cellular="" membrane,="" where="" collections="" of="" caveolae="" were="" identifiable,="" sr="" was="" thought="" out="" peripheral="" when="" situated="" in="" the="" collections="" of="" caveolae.="" informations="" represent="" mean="" ±="" search="" engine="" of="" the="" 8="" bladders.="" the="" sector="" of="" plasmalemma="" intruded="" by="" collections="" of="" big="" particles="" was="" analyzed="" trying="" the="" countrywide="" institutes="" of="" health="" photo="">
RESULTS
Western blotting for DHPR within the urinary bladder
The nonspecific response at high molecular weights in lane 1 for the bunnie small section is as a result of the utilization of crude lamb antisera made against a bunnie skeletal brawn preparation enriched in DHPR, in merger with a bunnie anti-sheep subsidiary antibody, on bunnie skeletal brawn membranes. This nonspecific response ain't witnessed with the guinea pig preparation. Other nonspecific bands are described within the fact legend.
hire car in malaga Immunofluorescence
Electron microscopy
Slim sections
Freeze-fracture
The 2 models of membrane domains (with and without caveolae) described beyond are explicitly identifiable in freeze-fracture imitations, where the openings of caveolae are observable as petite goes around (Fig. 5 A). Caveolae are disposed in membrane domains having the contour of stripes that appears to be oriented either longitudinally or at a bit of a angle about the longitudinal axis of the cellular and occupy ~50% of the exact amount surface sector. Caveolar domains are broke up from each other by longitudinal stripes of silky membrane without any invaginations, that correspond about the whereabouts of dense bodies.
Dialog
Immunolabeling and electron microscopy of silky brawn cells within the guinea pig urinary bladder offer complementary substantiation for the attendance of peripherally positioned complexes who have three important elements of the Ca^sup 2+^ cycle: the L-type Ca^sup 2+^ channels, DHPRs, that initiate e-c coupling; the RyRs, chargeable for Ca^sup 2+^ divulge from inside the SR; and calsequestrin, that enhances the over all SR storing potency for luminal Ca^sup 2+^., and which the sites with these components locate at or near to the cellular outer edge. Electron microscopy photos confirm the existence of JSR vesicles comprising toes and a dense content (purportedly calsequestrin) that appears to be needless to say present right at the surface membrane and form peripheral couplings with it. Toes are described as RyRs as a result of their size and to their whereabouts at the fiber outer edge in letter of the RyR-positive foci recognized by immunolabeling. The cytoplasmic domains of IP3 sensors are currently smaller than those of RyRs, although they have the equivalent four leaf clover appearance (Katayama et al., 1996). On this basis, realization of the toes with IP3 sensors may just be eliminated.
.;.; and Countrywide Institutes of Health grant RO1 HL-48093 to C..
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REFERENCES
Arikkath, J., C. C. Chen, C. Ahern, V. Allamand, J. D. Flanagan, R. Coronado, R. G. Gregg, and K. P. Campbell. 2003. Gamma 1 subunit interactions in the skeletal brawn L-type voltage-gated calcium channels. J. BM. Chem. 278:1212-1219.
Benham, C. D., and T. B. Bolton. 1986. Spontaneous transient outward currents in singular visceral and vascular silky brawn cells of the bunnie. J. Physiol. 381:385-406.
Block, B. A., T. Imagawa, K. P. Campbell, and C. Franzini-Armstrong. 1988. Structural substantiation for lead interplay amidst the molecular items of the transverse tubules/sarcoplasmic reticulum junction in skeletal brawn. J. Cellular Biol. 107:2587-2600.
Bolton, T. B., and D. V. Gordienko. 1998. Confocal imagining of calcium divulge ceremonies in singular silky brawn cells. Acta Physiol. Scand. 164:567-575.
Bolton, T. B., S. A. Prestwic, A. V. Zholos, and D. V. Gordienko. 1999. Excitation-contraction coupling in gastrointestinal and other silky muscle. Annu. Rev. Physiol. 61:85-115.
Interconnection, M., T. Kitazawa, A. P. Somlyo, and A. V. Somlyo. 1984. Divulge and recycling of calcium by the sarcoplasmic reticulum in guinea-pig gateway vein silky brawn. J. Physiol. 355:677-695.
Bozler, E. 1969. Role of calcium in initiation of activity of silky brawn. Am. J. Physiol. 216:671-674.
Cala, S. E., and L. R. Jones. 1983. Swift refinement of calsequestrin from heart failure and skeletal brawn sarcoplasmic reticulum vesicles by Ca^sup 2+^-dependent elution from phenyl-sepharose. J. Biol. Chem. 258: 11932-11936.
Campbell, K. R., C. Franzini-Armstrong, and A. E. Shamoo. 1980. Further characterization of light and heavy sarcoplasmic reticulum vesicles. Realization of the 'sarcoplasmic reticulum feet' linked with heavy sarcoplasmic reticulum vesicles. Biochim. Biophys. Ada. 602:97-116.
Carl, S. L., K. Felix, A. H. Caswell, N. R. Brandt, J. P. Brunschwig, G. Meissner, and D. G. Ferguson. 1995. Immunolocalization of triadin, DHP sensors, and ryanodine sensors in adult and developing skeletal brawn of rodents. Brawn Neurological. 18:1232-1243.
Del Valle-Rodriguez, A., J. Lopez-Barneo, and J. Urena. 2003. Ca^sup 2+^ channel-sarcoplasmic reticulum coupling: a appliances of arterial myocyte contraction without Ca^sup 2+^ inflow. EMBO J. 22:1-9.
Devine, C. E., A. V. Somlyo, and A. P. Somlyo. 1972. Sarcoplasmic reticulum and excitation contraction coupling in mammalian silky brawn. J. Cellular Biol. 52:690-718.
. F., and C. Franzini-Armstrong. 1975. The family member donations of folds and caveolae about the surface membrane of frog skeletal brawn fibers at distinct sarcomere lengths. J. Physiol. 250:513-539.
Ertel, E. A., K. P. Campbell, M. M. Harold, F. Hofmann, Y. Mori, E. Perez-Reyes, A. Schwartz, T. P. Snutch, T. Tanabe, L. Birnbaumer, R. W. Tsien, and W. A. Catterall. 2000. Nomenclature of voltage-gated calcium channels. Neuron. 25:533-535.
Etter, E. F., K. D. Philipson, W. A. Carrington, K. E. Fogarty, L. M. Lifshitz, and F. S. Fay. 1993. Coupling of the Na+/Ca^sup 2+^ exchanger, Na+/K+ pump and sarcoplasmic reticulum in silky brawn. Mother nature. 365:657-660.
Fay, F. S. 1995. Calcium triggers in vascular silky brawn: meditation government bodies. Science. 270:588-589.
Franzini-Armstrong, C., and C. W. Kish. 1995. Alternate tendency of tetrads in peripheral couplings of skeletal brawn. J. Brawn Res. Cellular Motil. 16:319-324.
Franzini-Armstrong, C., and F. Protasi. 1997. The ryanodine receptor of striated muscle, a complicated channel able of multi interactions. Physiol. Rev. 77:699-729.
Franzini-Armstrong, C., V. Ramesh, and F. Protasi. 1999. Shapes, dimensions and distributions of Ca^sup 2+^ divulge units and couplons in a number of skeletal and heart failure muscle. Biophys. J. 77:1528-1539.
Gabella, G. 1971. Caveolae intracellulares and sarcoplasmic reticulum in silky brawn. J. Cellular Sci. 8:601-609.
Gabella, G. 1972. The arrangement of sarcoplasmic reticulum in silky brawn. Experientia. 28:948-949.
Ganitkevich, V. Y., and G. Isenberg. 1990. Contribution of 2 models of calcium channels to membrane conductance of singular myocytes from guinea-pig coronary blood veins. J. Physiol. 426:19-42.
Ganitkevich, V. Y., and G. Isenberg. 1991. Depolarization-mediated intracellular calcium transients in isolated silky brawn cells of guinea-pig urinary bladder. J. Physiol. 435:187-205.
Ganitkevich, V. Y., and G. Isenberg. 1992. Contribution of Ca^sup 2+^-induced Ca^sup 2+^ divulge about the [Ca^sup 2+^]I transients in myocytes from guinea-pig urinary bladder. J. Physiol. 458:119-137.
Gerster, U., B. Neuhuber, K. Groschner, J. Striessnig, and B. E. Flucher. 1999. Existing modulation and membrane aiming for of the calcium channel alpha1C subunit are independent functions of the beta subunit. J. Physiol. 517:353-368.
Gordienko, D. V., T. B. Bolton, and M. B. Cannell. 1998. Variability in spontaneous subcellular calcium divulge in guinea-pig ileum silky brawn cells. J. Physiol. 507:707-720.
Gordienko, D. V., I. A, Greenwood, and T. B. Bolton. 2001. Lead visual images of sarcoplasmic reticulum specific zones discharging Ca^sup 2+^ triggers in vascular myocytes. Cellular Calcium. 29:13-28.
Gregg, R. G., A. Messing, C. Strube, M. Beurg, R. Moss, M. Behan, M. Sukhareva, S. Haynes, J. A. Powell, R. Coronado, and P. A. Energies. 1996. Lack of the [beta] subunit (cchb1) of the skeletal brawn dihydropyridine receptor changes expression of the al subunit and removes excitation-contraction coupling. Proc. Natl. Acad Sci. U . s .. 93: 13961-13966.
Herrera, G. M., T. J. Heppner, and M. T. Nelson. 2001. Voltage dependancy of the coupling of Ca^sup 2+^ triggers to BkCa channels in urinary bladder silky brawn. Am. J. Physiol. Cellular Physiol. 280:C481-C489.
Herrmann-Frank, A., E. Sweetheart, and G. Meissner. 1991. Functional characterization of the Ca(^sup 2+^)-gated Ca^sup 2+^ divulge channel of vascular silky brawn sarcoplasmic reticulum. Pflugers Archiv. 418:353-359.
Hohaus, A., M. Poteser, C. Romanin, N. Klugbauer, F. Hofmann, I. Morano, H. Haase, and K. Groschner. 2000. Modulation of the sleek L-type Ca^sup 2+^ channel alpha1 subunit (alpha1C-b) by the beta2a subunit: a peptide that inhibits bonding of beta about the I-II linker of alpha1 induces functional uncoupling. Biochem. J. 348:657-665.
Isenberg, G.,. Wendt-Gallitelli, and V. Y. Ganitkevich. 1992. Contribution of Ca^sup 2+^-induced Ca^sup 2+^-release to depolarization-induced Ca^sup 2+^ transients of myocytes from guinea-pig urinary bladder myocytes. Jpn. J. Pharmacol. 59:81-86.
Jaggar. J. H., V. A. Porter, W. J. Lederer, and M. T. Nelson. 2000. Calcium triggers in silky brawn. Am. J. Physiol. 278:235-256.
Jaggar, J. H., G. C. Wellman, T. J. Heppner, V. A. Porter, G. J. Perez, M. Gollasch, T. Kleppisch, M. Rubart, A. S. Stevenson, W. J. Lederer, H. J. Knot, A. D. Bonev, and M. T. Nelson. 1998. Ca^sup 2+^ channels, ryanodine sensors and Ca(2+)-activated K+ channels: a practical car rental malaga unit for regulating arterial tone. Acta Physiol. Scand. 164:577-578.
Jiang, D., B. Xiao, X. Li, and S. R. W. Chen. 2003. Silky your muscle mass express a chief dominant-negative splice variant of the kind 3 Ca^sup 2+^ divulge channel (Ryanodine receptor). J. Biol. Chem. 278:4763-4769.
Jorgensen, A. O., A. C. Shen, K. P. Campbell, and D. H. MacLennan. 1983. Ultrastructural localization of calsequestrin in mice skeletal brawn by immunoferritin denoting of ultrathin iced sections. J. Cellular Biol. 97: 1573-1581.
Junker, J., J. R. Sommer, M. Sar, and G. Meissner. 1994. Stretched out junctional sarcoplasmic reticulum of bird heart failure brawn includes functional ryanodine sensors. J. Biol. Chem. 269:1627-1634.
Katayama, E., H. Funahashi, T. Michikawa, T. Shiraishi, T. Ikemoto, M. Iino, and K. Mikoshiba. Indigenous structure and arrangement of inositol-1,4,5-trisphopshate receptor molecules in bovine cerebellar Purkinje cells as studied by quick-freeze deep-etch electron microscopy. EMBO J. 15:4844-4851.
Kirber, M. T., E. F. Euer, K. A. Bellve, L. M. Lifshitz, R. A. Tuft, F. S. Fay, J. V. Walsh, and K. E. Fogarty. 2001. Correlation of Ca^sup 2+^ triggers to STOCs studied with 2D and Three dimensional imagining in cat esophageal silky brawn cells. J. Physiol. 531:315-327.
Klöckner, U., and G. Isenberg. 1985a. Action possibilities and web membrane currents in isolated silky brawn cells (urinary bladder of the guinea-pig). Pflügers. Archiv. 405:329-339.
Klöckner, U., and G. Isenberg. 1985b. Calcium currents of cesium loaded isolated silky brawn cells (urinary bladder of the guinea-pig). Pflügers. Archiv. 405:340-348.
Klöckner, U., and G. Isenberg. 1991. Myocytes isolated from porcine coronary blood veins: elimination of currents through L-type Ca-channels by verapamil-type Ca-antagonists. J. Physiol. Pharmacol. 42:163-179.
Klöckner, U., and G. Isenberg. 1992. ATP repress activity of Ca^sup 2+^-activated K+ channels by Ca^sup 2+^ chelation. Pflügers. Archiv. 420: 101-105.
Klöckner, U., and G. Isenberg. 1994. Intracellular pH modulates the provision of vascular L sort Ca^sup 2+^ channels. J. Gen. Physiol. 103: 647-663.
Kockskämper, J., K. A. Sheehan, D. J. Naked, S. L. Lipsius, G. A. Mignery, and L. A. Blatter. 2001. Arousal and Propagation of Ca^sup 2+^ Divulge all through Excitation-Contraction Coupling in Atrial Myocytes. Biophys. J. 81:2590-2605.
Lai, F. A., Q. Y. Liu, L. Xu, A. el-Hashem, N. R. Kramarcy, R. Sealock, and G. Meissner. 1992. Amphibian ryanodine receptor isoforms are relevant to those of mammalian skeletal or heart failure brawn. Am. J. Physiol. 263:C545-C550.
Lesh, R. E., G. F. Nixon, S. Fleischer, J. A. Airey, A. P. Somlyo, and A. V. Somlyo. 1998. Localization of ryanodine sensors in silky brawn. Circ. Res. 8:175-185.
Leung, A. T., T. Imagawa, B. Block, C. Franzini-Armstrong, and K. P. Campbell. 1988. Biochemical and ultrastructural characterization of the 1,4-Dihydropyridine receptor from bunnie skeletal brawn. Substantiation for a 52,000 subunit. J. Biol Chem. 263: 994-1001.
MacLennan, D. H., and Wong, P. T. 1971. Solitude of a calcium-sequestering protein from sarcoplasmic reticulum. Proc. Natl. Acad. Sci. U . s .. 68:1231-1235.
Mahony, L., and L. R. Jones. 1986. Developmental alters in heart failure sarcoplasmic reticulum in lamb. J. Biol. Chem. 261:15257-15265.
Meissner, G. 1975. Solitude and characterization of 2 models of sarcoplasmic reticulum vesicles. Biochim. Biophys. Acta. 389:51-68.
Mironneau, J., S. Arnaudeau, N. Macrez-Lepretre, and F. X. Boittin. 1996. Ca^sup 2+^ triggers and Ca^sup 2+^ waves initialize distinct Ca^sup 2+^-dependent ion channels in singular myocytes from mice gateway vein. Cellular Calcium. 20: 153-160.
Moore, E. D. W., E. F. Etter, K. D. Philipson, W. A. Carrington, K. E. Fogarty, L. M. Lifshitz, and F. S. Fay. 1993. Coupling of the Na+/Ca^sup 2+^ exchanger, Na+/K+ pump in silky brawn. Mother nature. 365:657-660.
Morad, M., and L. Cleemann. 1987. Role of Ca^sup 2+^ channels in development of stress in heart brawn. J. Mol. Cellular. Cardiol 19:527-533.
Nakai, J., T. Tanabe, T. Konno, B. Adams, and K. G. B. Beam. 1998. Localization in the II-III loop of the dihydropyridine receptor of a chain critical for excitation-contraction coupling. J. Biol. Chem. 273:24983-24986.
Nelson, M. T., H. Cheng, M. Rubart, F. S. Santana, A. D. Bonev, H. J. Knot, and W. J. Lederer. 1995. Meditation of arterial silky malaga airport cars brawn by calcium triggers. Science. 270:633-637.
Neuhuber, B., U. Gerster, F. Doring, H. Glossmann, T. Tanabe, and B. E. Flucher. 1998. Association of calcium channel [alpha]1s and [beta]1a subunits is going to need for the aiming for of beta1a but not of alpha 1S into skeletal brawn triads. Proc. Natl. Acad. Sci. U . s .. 95:5015-5020.
Nixon, G. F., G. A. Mignery, and A. V. Somlyo. 1994. Immunogold localization of inositol 1,4,5-trisphosphate sensors and characterization of ultrastructural aspects of the sarcoplasmic reticulutn in phasic and tonic silky brawn. J. Brawn Res. Cellular Motil. 15:682-700.
Northern, A. J., B. Galazkiewicz, T. J. Byers, J. R. Glenney, and J. V. Petite. 1993. Complementary distributions of vinculin and dystrophin characterize two different sarcolemma domains in silky brawn. J. Cellular Biol. 120:1159-1167.
Ohi, Y., H. Yamamura, N. Nagno, M. Katsuhico, M. Watanabe, and Y. Imaizumi. 2003. Regional Ca^sup 2+^ transients and dispersion of BK channels and ryanodine sensors in silky brawn cells of guinea pig vas derens and urinary bladder. J. Physiol. 534:313-326.
Pérez, G. J., A. D. Bonev, J. B. Patlak, and M. T. Nelson. 1999. Functional coupling of ryanodinereceptors to KCa channels in silky brawn cells from mice cerebral blood veins. J. Gen. Physiol. 113:229-237.
Pragnell. M., J. Sakamoto, S. D. Jay, and K. P. Campbell. 1991. Cloning and tissue-specific expression of the brain calcium channel beta-subunit. FEBS Lett. 291:253-258.
Protasi, F., C. Franzini-Armstrong, and P. D. Allen. 1998. Role of ryanodine sensors within the assembly of calcium divulge units in skeletal brawn. J. Cellular Biol. 140:831-842.
Reimer, D., I. G. Huber, M. L. Garcia, H. Haase, and J. Striessnig. 2000. Beta subunit heterogeneity of L-type Ca^sup 2+^ channels in silky your muscle mass. FEBS Lett. 467:65-69.
Sato, T. 1968. A adapted means for direct staining of skinny sections. J. Electron Microsc. 17:158-159.
Schneider, P., H. H. Hopp, and G. Isenberg. 1991. Ca^sub 2+^ inflow through ATP-gated channels increments [Ca^sup 2+^]i and inactivates ICa in myocytes from guinea-pig urinary bladder. J. Physiol. 440:479-496.
Scriven, D. R. L., P. Dan, and E. D. W. Moore. 2000. Dispersion of amino acids implicated in excitation-contraction coupling in mice ventricular myocytes. Biophys. J. 79:2682-2691.
Somlyo, A. P., C. E. Devine, A. V. Somlyo, and S. R. Northern. 1971. Sarcoplasmic reticulum and the temperature-dependent contraction of silky brawn in calcium-free resolutions. J. Cellular Biol. 51:722-741.
Somlyo, A. V., and C. Franzini-Armstrong. 1985. New vistas of silky brawn structure exploiting icy, deep-etching and rotary shadowing. Experientia. 41:841-856.
Somlyo, A. P., and A. V. Somlyo. 1970. Vascular silky brawn. II. Pharmacology of ordinary and hypotensive vessels. Pharmacol. Rev. 22: 249-353.
Somlyo, A. P., and A. V. Somlyo. 1994. Signal transduction and legislation in silky brawn. Mother nature. 372:231-236.
Somlyo, A. P., J. W. Runner, Y. E. Goldman, D. R. Trentham, S. Kobayashi, T. Kitazawa, and A. V. Somlyo. 1988. Inositol trisphosphate, calcium and brawn contraction. Phil. Trans. Roy. Soc. London B. 320:399-414.
Sun,., F. Protasi, M. Takahashi, H. Takeshima, D. G. Ferguson, and C. Franzini-Armstrong. 1995. Molecular architecture of membranes engaged in excitation-contraction coupling of heart failure brawn. J. Cellular Biol. 129:659-673.
Sutko, J. L., and J. A. Airey. 1996. Ryanodine receptor Ca^sup 2+^ divulge channels: does variation in form equal variation in function? Physiol. Rev. 76:1027-1071.
Takekura, H., L. Bennett, T. Tanabe, K. G. Beam, and C. FranziniArmstrong. 1994. Retrieval of junctional tetrads in dysgenic myotubes by dihydropyridine receptor cDNA. Biophys. J. 67:793-804.
Tijskens, P., G. Meissner, and C. Franzini-Armstrong. 2003. Whereabouts of ryanodine and dihydropyridine sensors in frog myocardium. Biophys. J. 84:1079-1092.
Villa, A., P. Podini, M. C. Panzeri, H. D. Soling, P. Volpe, and J. Meldolesi. 1993. The endoplasmic-sarcoplasmic reticulum of silky brawn: immunocytochemistry of vas deferens fibres uncovers specialised subcompartments in a different way furnished for the control of calcium homeostasis. J. Cellular Biol. 121:1041-51.
Wang, S. Q., M. D. Stringent, E. Rios, and H. Cheng. 2004. The quantal mother nature of Ca^sup 2+^ triggers and in situ operation of ryanodine receptor assortment in heart failure cells. Proc. Natl. Acad. Sci U . s .. low cost malaga car rental 101:3979-3984.
Weibel, E. R. 1979. Stereological Ways and means,. Tutorial Squeeze, Ny.
Wuytack, F., L. Raeymaekers, J. Verbist, L. R. Jones, and R. Casteels. 1987. Smooth-muscle endoplasmic reticulum posesses a cardiac-like shape of calsequestrin. Biochim. Biophys. Acta. 899:151-158.
Xu, L., F. A. Lai, A. Cohn, E. Etter, A. Guerrero, F. S. Fay, and G. Meissner. 1994. Substantiation for a Ca(2+)-gated ryanodine-sensitive Ca^sup 2+^ divulge channel in visceral silky brawn. Proc. Nail. Acad. Sci. U . s .. 91:3294-3298.
ZhuGe, R., K. E. Fogarty, R. A. Tuft, and J. V. Walsh Jr. 2002. Spontaneous transient outward currents pop up from microdomains where BK channels are disclosed to a mean Ca^sup 2+^ attention to the order of 10 microM all through a Ca^sup 2+^ twinkle. J. Gen. Physiol. 120:15-27.
ZhuGe, R., S. M. Sims, R. A. Tuft, K. E. Fogarty, and J. V. Walsh Jr. 1998. Ca^sup 2+^ triggers initialize K+ and Cl- channels, resulting in spontaneous transient currents in guinea-pig tracheal myocytes. J. Physiol. 513: 711-718.
ZhuGe, R., R. A. Tuft, K. E. Fogarty, K. Bellve, F. S. Fay, and J. V. Walsh Jr. 1999. The influence of sarcoplasmic reticulum Ca^sup 2+^ attention to Ca^sup 2+^ triggers and spontaneous transient outward currents in singular silky brawn. J. Gen. Physiol. 113:215-228.
[Author Network]
Edwin D. Moore,* Tilman Voigt,[dagger] Yvonne M. Kobayashi,[double knife] Gerrit Isenberg,[dagger] Fred S. Fay,¶ Maria F. Gallitelli,[dagger] and Clara Franzini-Armstrong§
* Dept of Physiology, College of Brit Columbia, Vancouver, Brit Columbia, Canada; [knife] Julius-Bernstein Institut für Physiologie, College of Halle, D-06097 Halle, Germany; [double knife] Howard Hughes Medicinal Institute, Dept of Physiology and Biophysics, College of Iowa, Iowa City, Iowa, U . s .; ¶ Dept of Physiology and Biomedical Imagining Team, College of Massachusetts Medicinal School, Worcester, Massachusetts, U . s . (departed); and § Dept of Cellular and Development Biology, College of Pennsylvania, Philadelphia, Pennsylvania, U . s .
[Author Network]
Submitted April 6, 2004, and approved for e-newsletter June 22, 2004.
Address reprint orders to Clara Franzini-Armstrong, B1 Anatomy-Chemistry Constructing, Division. of Cellular and Developmental Biology, College of Pennsylvania, Philadelphia, PA 19104-6058.;;.
